Molecular Cloning and Expression of Abscisic Acid-Responsive Genes in Embryos of Dormant Wheat Seeds

temporal changes in abscisic acid concentration in dormant and non-dormant seeds of wheat (triticum spp.) genotypes

little is known about the levels and physiological role of endogenous abscisic acid (aba) during after-ripening and germination. genetic variants for aba content were investigated to account for the role of aba in the persistence of seed dormancy. in this study, genotypic variation in aba contents at zadok’s growth stage 92 (zgs 92), temporal changes in aba during two physiological stages (...

Synthesis of abscisic Acid-responsive, heat-stable proteins in embryonic axes of dormant wheat grain.

Germination of embryonic axes from dormant grain is inhibited by low concentrations of abscisic acid (ABA) compared with axes from nondormant grain. Incubation of dormant grain axes in 0.05 to 50 micromolar ABA caused the prolonged synthesis of a set of heat-stable proteins. Two of these proteins were identified as dehydrins. In nondormant grain axes, 100- to 1000-fold greater ABA concentration...

Abscisic acid-responsive sequences from the em gene of wheat.

We demonstrate that a chimeric gene containing the beta-glucuronidase (GUS) reporter gene linked to a 646-base pair 5' fragment (-554 to +92) from the abscisic acid (ABA)-regulated Em gene from wheat is correctly expressed in transgenic tobacco. We observe high activity only in embryos of mature seeds, and immature seeds cultured on ABA show enhanced expression. Using a rice transient assay, we...

molecular and physiological characterizations of floral scent production in rosa damascena mill.

گل محمدی (r. damascena mill.)مهمترین گونه ای است که در تولید گلاب، عطر و اسانس، در صنعت عطرسازی و دارویی کاربرد دارد. با وجود اهمیت عطر گل محمدی، پژوهش های مولکولی و بیوشیمیایی عطر گل این گونه هنوز در مراحل ابتدایی است. در این پژوهش، ویژگی های مولکولی و فیزیولوژیکی تولید عطر در گل محمدی در آزمایش های مختلف مورد بررسی قرار گرفتند. در آزمایش اول، پژوهشی به منظور بررسی تنوع شیمیایی اسانس میان 44 ت...

Abscisic acid signaling in seeds and seedlings.